trypsin solution without edta Search Results


trypsin edta  (ATCC)
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ATCC trypsin edta
Trypsin Edta, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trypsin edta  (GE Healthcare)
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GE Healthcare trypsin edta
Trypsin Edta, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trypsin edta solution  (Valiant Co Ltd)
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Valiant Co Ltd trypsin edta solution
Trypsin Edta Solution, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phenol red  (Valiant Co Ltd)
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Valiant Co Ltd phenol red
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trypsin edta  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology trypsin edta
Trypsin Edta, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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incubation in trypsin  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology incubation in trypsin
Prolonged effect of ATF3 inhibition on neurite outgrowth. ( A1 – A4 ) Cortical neurons derived from P5 opossum. ( A1 , A3 ) represent control neuronal cultures treated with PBS only. At DIV10, neurons were first treated with SP inhibitor for 24 h, followed by scratch and washout for the next 24 h ( A2 ), or <t>incubated</t> with SP immediately after scratch over the next 24 h and followed by 24 h washout ( A4 ). ( B1 – B4 ) Cortical neurons derived from P16 opossum. ( B1 , B3 ) represent control neuronal cultures treated with PBS only. At DIV10, neurons were first treated with SP inhibitor for 24 h, followed by scratch and washout for the next 24 h ( B2 ), or incubated with SP immediately after scratch over the next 24 h and followed by 24 h washout ( B4 ). Neurons were fixed and immunostained for TUJ1. The scratch area is defined by the red dashed lines. Scale bar is 50 μm. ( C ) The scatter plot shows P5 average TUJ1 pixel intensity. Data are shown as mean ± SD. For each condition, 2 ROIs of 3 random fields per sample from 3 culture preparations were analyzed. Welch’s t -test, SHAM CUT 24H vs. SP + CUT 24H p < 0.001 ***. Unpaired t -test, SHAM CUT 48H vs. CUT 48H + SP p < 0.001 ***. ( D ) The scatter plot shows P16 average TUJ1 pixel intensity. Data are shown as mean ± SD. For each condition, 2 ROIs of 3 random fields per sample from 3 culture preparations were analyzed. Unpaired t -test, SHAM CUT 24H vs. SP + CUT 24H p < 0.001 ***. Unpaired t -test, SHAM CUT 48H vs. CUT 48H + SP p < 0.001 ***. ( E ) Schematic representation of the time course of SP inhibitor application. ( 1 ) represents the application of SP for experimental conditions ( A2 , B2 ). ( 2 ) represents the application of SP inhibitor for experimental conditions ( A4 , B4 ).
Incubation In Trypsin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trypsin  (GE Healthcare)
92
GE Healthcare trypsin
Prolonged effect of ATF3 inhibition on neurite outgrowth. ( A1 – A4 ) Cortical neurons derived from P5 opossum. ( A1 , A3 ) represent control neuronal cultures treated with PBS only. At DIV10, neurons were first treated with SP inhibitor for 24 h, followed by scratch and washout for the next 24 h ( A2 ), or <t>incubated</t> with SP immediately after scratch over the next 24 h and followed by 24 h washout ( A4 ). ( B1 – B4 ) Cortical neurons derived from P16 opossum. ( B1 , B3 ) represent control neuronal cultures treated with PBS only. At DIV10, neurons were first treated with SP inhibitor for 24 h, followed by scratch and washout for the next 24 h ( B2 ), or incubated with SP immediately after scratch over the next 24 h and followed by 24 h washout ( B4 ). Neurons were fixed and immunostained for TUJ1. The scratch area is defined by the red dashed lines. Scale bar is 50 μm. ( C ) The scatter plot shows P5 average TUJ1 pixel intensity. Data are shown as mean ± SD. For each condition, 2 ROIs of 3 random fields per sample from 3 culture preparations were analyzed. Welch’s t -test, SHAM CUT 24H vs. SP + CUT 24H p < 0.001 ***. Unpaired t -test, SHAM CUT 48H vs. CUT 48H + SP p < 0.001 ***. ( D ) The scatter plot shows P16 average TUJ1 pixel intensity. Data are shown as mean ± SD. For each condition, 2 ROIs of 3 random fields per sample from 3 culture preparations were analyzed. Unpaired t -test, SHAM CUT 24H vs. SP + CUT 24H p < 0.001 ***. Unpaired t -test, SHAM CUT 48H vs. CUT 48H + SP p < 0.001 ***. ( E ) Schematic representation of the time course of SP inhibitor application. ( 1 ) represents the application of SP for experimental conditions ( A2 , B2 ). ( 2 ) represents the application of SP inhibitor for experimental conditions ( A4 , B4 ).
Trypsin, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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angio proteomie trypsin edta solution cap  (Angio-Proteomie)
86
Angio-Proteomie angio proteomie trypsin edta solution cap
Prolonged effect of ATF3 inhibition on neurite outgrowth. ( A1 – A4 ) Cortical neurons derived from P5 opossum. ( A1 , A3 ) represent control neuronal cultures treated with PBS only. At DIV10, neurons were first treated with SP inhibitor for 24 h, followed by scratch and washout for the next 24 h ( A2 ), or <t>incubated</t> with SP immediately after scratch over the next 24 h and followed by 24 h washout ( A4 ). ( B1 – B4 ) Cortical neurons derived from P16 opossum. ( B1 , B3 ) represent control neuronal cultures treated with PBS only. At DIV10, neurons were first treated with SP inhibitor for 24 h, followed by scratch and washout for the next 24 h ( B2 ), or incubated with SP immediately after scratch over the next 24 h and followed by 24 h washout ( B4 ). Neurons were fixed and immunostained for TUJ1. The scratch area is defined by the red dashed lines. Scale bar is 50 μm. ( C ) The scatter plot shows P5 average TUJ1 pixel intensity. Data are shown as mean ± SD. For each condition, 2 ROIs of 3 random fields per sample from 3 culture preparations were analyzed. Welch’s t -test, SHAM CUT 24H vs. SP + CUT 24H p < 0.001 ***. Unpaired t -test, SHAM CUT 48H vs. CUT 48H + SP p < 0.001 ***. ( D ) The scatter plot shows P16 average TUJ1 pixel intensity. Data are shown as mean ± SD. For each condition, 2 ROIs of 3 random fields per sample from 3 culture preparations were analyzed. Unpaired t -test, SHAM CUT 24H vs. SP + CUT 24H p < 0.001 ***. Unpaired t -test, SHAM CUT 48H vs. CUT 48H + SP p < 0.001 ***. ( E ) Schematic representation of the time course of SP inhibitor application. ( 1 ) represents the application of SP for experimental conditions ( A2 , B2 ). ( 2 ) represents the application of SP inhibitor for experimental conditions ( A4 , B4 ).
Angio Proteomie Trypsin Edta Solution Cap, supplied by Angio-Proteomie, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dissociation reagent trypsin-edta 1x solution containing 0.05% trypsin and 0.02% edta  (TIBCO)
90
TIBCO dissociation reagent trypsin-edta 1x solution containing 0.05% trypsin and 0.02% edta
Prolonged effect of ATF3 inhibition on neurite outgrowth. ( A1 – A4 ) Cortical neurons derived from P5 opossum. ( A1 , A3 ) represent control neuronal cultures treated with PBS only. At DIV10, neurons were first treated with SP inhibitor for 24 h, followed by scratch and washout for the next 24 h ( A2 ), or <t>incubated</t> with SP immediately after scratch over the next 24 h and followed by 24 h washout ( A4 ). ( B1 – B4 ) Cortical neurons derived from P16 opossum. ( B1 , B3 ) represent control neuronal cultures treated with PBS only. At DIV10, neurons were first treated with SP inhibitor for 24 h, followed by scratch and washout for the next 24 h ( B2 ), or incubated with SP immediately after scratch over the next 24 h and followed by 24 h washout ( B4 ). Neurons were fixed and immunostained for TUJ1. The scratch area is defined by the red dashed lines. Scale bar is 50 μm. ( C ) The scatter plot shows P5 average TUJ1 pixel intensity. Data are shown as mean ± SD. For each condition, 2 ROIs of 3 random fields per sample from 3 culture preparations were analyzed. Welch’s t -test, SHAM CUT 24H vs. SP + CUT 24H p < 0.001 ***. Unpaired t -test, SHAM CUT 48H vs. CUT 48H + SP p < 0.001 ***. ( D ) The scatter plot shows P16 average TUJ1 pixel intensity. Data are shown as mean ± SD. For each condition, 2 ROIs of 3 random fields per sample from 3 culture preparations were analyzed. Unpaired t -test, SHAM CUT 24H vs. SP + CUT 24H p < 0.001 ***. Unpaired t -test, SHAM CUT 48H vs. CUT 48H + SP p < 0.001 ***. ( E ) Schematic representation of the time course of SP inhibitor application. ( 1 ) represents the application of SP for experimental conditions ( A2 , B2 ). ( 2 ) represents the application of SP inhibitor for experimental conditions ( A4 , B4 ).
Dissociation Reagent Trypsin Edta 1x Solution Containing 0.05% Trypsin And 0.02% Edta, supplied by TIBCO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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0.25% trypsin-edta paneco  (PanEco Scientific)
90
PanEco Scientific 0.25% trypsin-edta paneco
Prolonged effect of ATF3 inhibition on neurite outgrowth. ( A1 – A4 ) Cortical neurons derived from P5 opossum. ( A1 , A3 ) represent control neuronal cultures treated with PBS only. At DIV10, neurons were first treated with SP inhibitor for 24 h, followed by scratch and washout for the next 24 h ( A2 ), or <t>incubated</t> with SP immediately after scratch over the next 24 h and followed by 24 h washout ( A4 ). ( B1 – B4 ) Cortical neurons derived from P16 opossum. ( B1 , B3 ) represent control neuronal cultures treated with PBS only. At DIV10, neurons were first treated with SP inhibitor for 24 h, followed by scratch and washout for the next 24 h ( B2 ), or incubated with SP immediately after scratch over the next 24 h and followed by 24 h washout ( B4 ). Neurons were fixed and immunostained for TUJ1. The scratch area is defined by the red dashed lines. Scale bar is 50 μm. ( C ) The scatter plot shows P5 average TUJ1 pixel intensity. Data are shown as mean ± SD. For each condition, 2 ROIs of 3 random fields per sample from 3 culture preparations were analyzed. Welch’s t -test, SHAM CUT 24H vs. SP + CUT 24H p < 0.001 ***. Unpaired t -test, SHAM CUT 48H vs. CUT 48H + SP p < 0.001 ***. ( D ) The scatter plot shows P16 average TUJ1 pixel intensity. Data are shown as mean ± SD. For each condition, 2 ROIs of 3 random fields per sample from 3 culture preparations were analyzed. Unpaired t -test, SHAM CUT 24H vs. SP + CUT 24H p < 0.001 ***. Unpaired t -test, SHAM CUT 48H vs. CUT 48H + SP p < 0.001 ***. ( E ) Schematic representation of the time course of SP inhibitor application. ( 1 ) represents the application of SP for experimental conditions ( A2 , B2 ). ( 2 ) represents the application of SP inhibitor for experimental conditions ( A4 , B4 ).
0.25% Trypsin Edta Paneco, supplied by PanEco Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trypsin/edta solution  (Kurabo industries)
90
Kurabo industries trypsin/edta solution
Prolonged effect of ATF3 inhibition on neurite outgrowth. ( A1 – A4 ) Cortical neurons derived from P5 opossum. ( A1 , A3 ) represent control neuronal cultures treated with PBS only. At DIV10, neurons were first treated with SP inhibitor for 24 h, followed by scratch and washout for the next 24 h ( A2 ), or <t>incubated</t> with SP immediately after scratch over the next 24 h and followed by 24 h washout ( A4 ). ( B1 – B4 ) Cortical neurons derived from P16 opossum. ( B1 , B3 ) represent control neuronal cultures treated with PBS only. At DIV10, neurons were first treated with SP inhibitor for 24 h, followed by scratch and washout for the next 24 h ( B2 ), or incubated with SP immediately after scratch over the next 24 h and followed by 24 h washout ( B4 ). Neurons were fixed and immunostained for TUJ1. The scratch area is defined by the red dashed lines. Scale bar is 50 μm. ( C ) The scatter plot shows P5 average TUJ1 pixel intensity. Data are shown as mean ± SD. For each condition, 2 ROIs of 3 random fields per sample from 3 culture preparations were analyzed. Welch’s t -test, SHAM CUT 24H vs. SP + CUT 24H p < 0.001 ***. Unpaired t -test, SHAM CUT 48H vs. CUT 48H + SP p < 0.001 ***. ( D ) The scatter plot shows P16 average TUJ1 pixel intensity. Data are shown as mean ± SD. For each condition, 2 ROIs of 3 random fields per sample from 3 culture preparations were analyzed. Unpaired t -test, SHAM CUT 24H vs. SP + CUT 24H p < 0.001 ***. Unpaired t -test, SHAM CUT 48H vs. CUT 48H + SP p < 0.001 ***. ( E ) Schematic representation of the time course of SP inhibitor application. ( 1 ) represents the application of SP for experimental conditions ( A2 , B2 ). ( 2 ) represents the application of SP inhibitor for experimental conditions ( A4 , B4 ).
Trypsin/Edta Solution, supplied by Kurabo industries, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Prolonged effect of ATF3 inhibition on neurite outgrowth. ( A1 – A4 ) Cortical neurons derived from P5 opossum. ( A1 , A3 ) represent control neuronal cultures treated with PBS only. At DIV10, neurons were first treated with SP inhibitor for 24 h, followed by scratch and washout for the next 24 h ( A2 ), or incubated with SP immediately after scratch over the next 24 h and followed by 24 h washout ( A4 ). ( B1 – B4 ) Cortical neurons derived from P16 opossum. ( B1 , B3 ) represent control neuronal cultures treated with PBS only. At DIV10, neurons were first treated with SP inhibitor for 24 h, followed by scratch and washout for the next 24 h ( B2 ), or incubated with SP immediately after scratch over the next 24 h and followed by 24 h washout ( B4 ). Neurons were fixed and immunostained for TUJ1. The scratch area is defined by the red dashed lines. Scale bar is 50 μm. ( C ) The scatter plot shows P5 average TUJ1 pixel intensity. Data are shown as mean ± SD. For each condition, 2 ROIs of 3 random fields per sample from 3 culture preparations were analyzed. Welch’s t -test, SHAM CUT 24H vs. SP + CUT 24H p < 0.001 ***. Unpaired t -test, SHAM CUT 48H vs. CUT 48H + SP p < 0.001 ***. ( D ) The scatter plot shows P16 average TUJ1 pixel intensity. Data are shown as mean ± SD. For each condition, 2 ROIs of 3 random fields per sample from 3 culture preparations were analyzed. Unpaired t -test, SHAM CUT 24H vs. SP + CUT 24H p < 0.001 ***. Unpaired t -test, SHAM CUT 48H vs. CUT 48H + SP p < 0.001 ***. ( E ) Schematic representation of the time course of SP inhibitor application. ( 1 ) represents the application of SP for experimental conditions ( A2 , B2 ). ( 2 ) represents the application of SP inhibitor for experimental conditions ( A4 , B4 ).

Journal: International Journal of Molecular Sciences

Article Title: The Role of ATF3 in Neuronal Differentiation and Development of Neuronal Networks in Opossum Postnatal Cortical Cultures

doi: 10.3390/ijms23094964

Figure Lengend Snippet: Prolonged effect of ATF3 inhibition on neurite outgrowth. ( A1 – A4 ) Cortical neurons derived from P5 opossum. ( A1 , A3 ) represent control neuronal cultures treated with PBS only. At DIV10, neurons were first treated with SP inhibitor for 24 h, followed by scratch and washout for the next 24 h ( A2 ), or incubated with SP immediately after scratch over the next 24 h and followed by 24 h washout ( A4 ). ( B1 – B4 ) Cortical neurons derived from P16 opossum. ( B1 , B3 ) represent control neuronal cultures treated with PBS only. At DIV10, neurons were first treated with SP inhibitor for 24 h, followed by scratch and washout for the next 24 h ( B2 ), or incubated with SP immediately after scratch over the next 24 h and followed by 24 h washout ( B4 ). Neurons were fixed and immunostained for TUJ1. The scratch area is defined by the red dashed lines. Scale bar is 50 μm. ( C ) The scatter plot shows P5 average TUJ1 pixel intensity. Data are shown as mean ± SD. For each condition, 2 ROIs of 3 random fields per sample from 3 culture preparations were analyzed. Welch’s t -test, SHAM CUT 24H vs. SP + CUT 24H p < 0.001 ***. Unpaired t -test, SHAM CUT 48H vs. CUT 48H + SP p < 0.001 ***. ( D ) The scatter plot shows P16 average TUJ1 pixel intensity. Data are shown as mean ± SD. For each condition, 2 ROIs of 3 random fields per sample from 3 culture preparations were analyzed. Unpaired t -test, SHAM CUT 24H vs. SP + CUT 24H p < 0.001 ***. Unpaired t -test, SHAM CUT 48H vs. CUT 48H + SP p < 0.001 ***. ( E ) Schematic representation of the time course of SP inhibitor application. ( 1 ) represents the application of SP for experimental conditions ( A2 , B2 ). ( 2 ) represents the application of SP inhibitor for experimental conditions ( A4 , B4 ).

Article Snippet: Enzyme digestion was performed by incubation in trypsin (0.5% Trypsin-EDTA Solution 10X, cat. no. sc-363354, Santa Cruz Biotechnology, SCBT, Dallas, TX, USA) at 32.5 °C.

Techniques: Inhibition, Derivative Assay, Control, Incubation